SubtiBank SubtiBank
ddcP [2018-10-15 13:14:40]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

ddcP [2018-10-15 13:14:40]

DNA damage checkpoint recovery protease, promotes DNA damage checkpoint recovery
locus
BSU15050
pI
5.85
mw
38.61 kDa
protein length
350 aa Sequence Blast
gene length
1050 bp Sequence Blast
function
degradation of [protein|7BF591DCDC9635C605D76135481A8A9DB63EE861|YneA]
product
DNA damage checkpoint recovery protease
essential
no
synonyms
ylbL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.4|Additional proteins involved in proteolysis]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,572,765 → 1,573,790

    Phenotypes of a mutant

  • deletion of [protein|5ED8301B9681FDD5171A9344A971E891493A6558|CtpA] and [protein|1A5C1BDE844AA56B90C0E4A02D74978676487E99|DdcP] leads to accumulation of the checkpoint protein [protein|7BF591DCDC9635C605D76135481A8A9DB63EE861|YneA] [pubmed|29979679]
  • sensitivity to DNA damage [pubmed|29979679]

    • The protein

    [SW|Domains]

  • TM helix (aa 9 - 29) [pubmed|29979679]
  • [SW|PDZ domain] (aa 100 - 186) [pubmed|29979679]
  • Lon protease domain (aa 187 - 336) [pubmed|29979679]

    • Structure

  • [PDB|2KJP] (PDZ domain)

    • [SW|Localization]

  • cell membrane [pubmed|30315724]

    • Expression and Regulation

    Operons

    genes
    [gene|DE3BCF20DE5BB438ACBB36B412817F6124CFDFFC|ylbK]-[gene|1A5C1BDE844AA56B90C0E4A02D74978676487E99|ddcP]
    description
    [pubmed|22383849]
    view in new tab

    genes
    [gene|1A5C1BDE844AA56B90C0E4A02D74978676487E99|ddcP]
    description
    [pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • MGNA-B249 (ylbL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1248 NBRP B. subtilis, Japan]
  • BKE15050 (Δ[gene|1A5C1BDE844AA56B90C0E4A02D74978676487E99|ddcP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCTAAAATGTTTTTTACGTA, downstream forward: _UP4_CTGAAAGCGAAAAGCACCTG
  • BKK15050 (Δ[gene|1A5C1BDE844AA56B90C0E4A02D74978676487E99|ddcP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCTAAAATGTTTTTTACGTA, downstream forward: _UP4_CTGAAAGCGAAAAGCACCTG

    • References

    Research papers

  • 29979679,30315724